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After being forced off with a hamstring in the 3-0 defeat to Bournemouth, Andreas Christensen will miss the trip to Watford.
But David Luiz and Willian were able to train on Thursday, and Antonio Conte was hopeful that Willian will be fit to return.
New signings Emerson Palmieri and Olivier Giroud may be in contention to make their debuts, although Conte hinted that Giroud might not be fit enough to start the match.
Antonio Rudiger should replace Christensen in central defence, while after being an unused substitute on Wednesday night, Victor Moses is in line to come in for Davide Zappacosta at right wing-back.
Palmieri is a possible alternative to Alonso at left wing-back, although he is short of match fitness and is not expected to push for a start.
Cesc Fabregas only played 36 minutes in midweek, while Danny Drinkwater was an unused substitute. So should Antonio Conte look to introduce some fresh legs, either player could replace Tiemoue Bakayoko in central midfield.
Alvaro Morata remains out with a back problem so Eden Hazard could start as a “false nine” flanked by Pedro and Willian.
Ross Barkley is expected to drop back to the bench.